Optimod--an automated approach for constructing and optimizing initial models for single-particle electron microscopy.

ABSTRACT: Single-particle cryo-electron microscopy is now well established as a technique for the structural characterization of large macromolecules and macromolecular complexes. The raw data is very noisy and consists of two-dimensional projections, from which the 3D biological object must be reconstructed. The 3D object depends upon knowledge of proper angular orientations assigned to the 2D projection images. Numerous algorithms have been developed for determining relative angular orientations between 2D images, but the transition from 2D to 3D remains challenging and can result in erroneous and conflicting results. Here we describe a general, automated procedure, called OptiMod, for reconstructing and optimizing 3D models using common-lines methodologies. OptiMod approximates orientation angles

SUBMITTER: Lyumkis D 

PROVIDER: S-EPMC3885246 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature