Thiols and selenols as electron-relay catalysts for disulfide-bond reduction.

ABSTRACT: Pass them on! Dithiobutylamine immobilized on a resin is a useful reagent for the reduction of disulfide bonds. Its ability to reduce a disulfide bond in a protein is enhanced greatly if used along with a soluble strained cyclic disulfide or mixed diselenide that relays electrons from the resin to the protein. This electron-relay catalysis system provides distinct advantages over the use of excess soluble reducing agent alone.

SUBMITTER: Lukesh JC 

PROVIDER: S-EPMC3885359 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature