Evaluation of microbial bacterial and fungal diversity in cerebrospinal fluid shunt infection.

ABSTRACT:

Background

Cerebrospinal fluid shunt infection can be recalcitrant. Recurrence is common despite appropriate therapy for the pathogens identified by culture. Improved diagnostic and therapeutic approaches are required, and culture-independent molecular approaches to cerebrospinal fluid shunt infections have not been described.

Objectives

To identify the bacteria and fungi present in cerebrospinal fluid from children with cerebrospinal fluid shunt infection using a high-throughput sequencing approach, and to compare those results to those from negative controls and conventional culture.

Methods

This descriptive study included eight children ≤18 years old undergoing treatment for culture-identified cerebrospinal fluid shunt infection. After routine aerobic culture of ea

SUBMITTER: Simon TD 

PROVIDER: S-EPMC3885436 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature