A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis.

ABSTRACT: Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins. The often used transient transformation methods included particle bombardment, protoplast transfection and Agrobacterium-mediated transformation. Particle bombardment in onion epidermis was successfully established, however, it was expensive, biolistic equipment dependent and with low transformation efficiency. We developed a highly efficient in planta transient transformation method in onion epidermis by u

SUBMITTER: Xu K 

PROVIDER: S-EPMC3885512 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature