Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling.

ABSTRACT:

Objectives

To determine the efficacy of perivascular delivery of Notch 1 siRNA in preventing injury-induced arterial remodeling.

Methods and results

Carotid artery ligation was performed to induce arterial remodeling. After 14 days, morphometric analysis confirmed increased vSMC growth and subsequent media thickening and neointimal formation. Laser capture microdissection, quantitative qRT-PCR and immunoblot analysis of medial tissue revealed a significant increase in Notch1 receptor and notch target gene, Hrt 1 and 2 expression in the injured vessels. Perivascular delivery of Notch 1 siRNA by pluronic gel inhibited the injury-induced increase in Notch 1 receptor and target gene expression when compared to scrambled siRNA controls while concomitantly reducing media thickenin

SUBMITTER: Redmond EM 

PROVIDER: S-EPMC3885538 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature