Prediction of detailed enzyme functions and identification of specificity determining residues by random forests.

ABSTRACT: Determining enzyme functions is essential for a thorough understanding of cellular processes. Although many prediction methods have been developed, it remains a significant challenge to predict enzyme functions at the fourth-digit level of the Enzyme Commission numbers. Functional specificity of enzymes often changes drastically by mutations of a small number of residues and therefore, information about these critical residues can potentially help discriminate detailed functions. However, because these residues must be identified by mutagenesis experiments, the available information is limited, and the lack of experimentally verified specificity determining residues (SDRs) has hindered the development of detailed function prediction methods and computational identification of SDRs. Here we

SUBMITTER: Nagao C 

PROVIDER: S-EPMC3885575 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature