A majority of human melanoma cell lines exhibits an S phase-specific defect in excision of UV-induced DNA photoproducts.

ABSTRACT: It is well established that efficient removal of highly-promutagenic UV-induced dipyrimidine photoproducts via nucleotide excision repair (NER) is required for protection against sunlight-associated malignant melanoma. Nonetheless, the extent to which reduced NER capacity might contribute to individual melanoma susceptibility in the general population remains unclear. Here we show that among a panel of 14 human melanoma strains, 11 exhibit significant inhibition of DNA photoproduct removal during S phase relative to G0/G1 or G2/M. Evidence is presented that this cell cycle-specific NER defect correlates with enhanced apoptosis and reduced clonogenic survival following UV irradiation. In addition, melanoma strains deficient in S phase-specific DNA photoproduct removal manifest significantly

SUBMITTER: Belanger F 

PROVIDER: S-EPMC3885708 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature