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Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease.

ABSTRACT: A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8-10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market.

SUBMITTER: Majeed T 

PROVIDER: S-EPMC3886229 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease.

Majeed Tanveer T   Tabassum Romana R   Orts William J WJ   Lee Charles C CC  

TheScientificWorldJournal 20131225

A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8-10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. pro  ...[more]

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