CD44 is a negative cell surface marker for pluripotent stem cell identification during human fibroblast reprogramming.

ABSTRACT: Induced pluripotent stem cells (iPSCs) are promising tools for disease research and cell therapy. One of the critical steps in establishing iPSC lines is the early identification of fully reprogrammed colonies among unreprogrammed fibroblasts and partially reprogrammed intermediates. Currently, colony morphology and pluripotent stem cell surface markers are used to identify iPSC colonies. Through additional clonal characterization, we show that these tools fail to distinguish partially reprogrammed intermediates from fully reprogrammed iPSCs. Thus, they can lead to the selection of suboptimal clones for expansion. A subsequent global transcriptome analysis revealed that the cell adhesion protein CD44 is a marker that differentiates between partially and fully reprogrammed cells. Immunohist

SUBMITTER: Quintanilla RH 

PROVIDER: S-EPMC3887044 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature