Golgi enrichment and proteomic analysis of developing Pinus radiata xylem by free-flow electrophoresis.

ABSTRACT: Our understanding of the contribution of Golgi proteins to cell wall and wood formation in any woody plant species is limited. Currently, little Golgi proteomics data exists for wood-forming tissues. In this study, we attempted to address this issue by generating and analyzing Golgi-enriched membrane preparations from developing xylem of compression wood from the conifer Pinus radiata. Developing xylem samples from 3-year-old pine trees were harvested for this purpose at a time of active growth and subjected to a combination of density centrifugation followed by free flow electrophoresis, a surface charge separation technique used in the enrichment of Golgi membranes. This combination of techniques was successful in achieving an approximately 200-fold increase in the activity of the Golgi

SUBMITTER: Parsons HT 

PROVIDER: S-EPMC3887118 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature