Ferredoxin Gene Mutation in Iranian Trichomonas vaginalis Isolates.

ABSTRACT:

Background

Trichomonas vaginalis causes trichomoniasis and metronidazole is its chosen drug for treatment. Ferredoxin has role in electron transport and carbohydrate metabolism and the conversion of an inactive form of metronidazole (CO) to its active form (CPR). Ferredoxin gene mutations reduce gene expression and increase its resistance to metronidazole. In this study, the frequency of ferredoxin gene mutations in clinical isolates of T.vaginalis in Tehran has been studied.

Methods

Forty six clinical T. vaginalis isolates of vaginal secretions and urine sediment were collected from Tehran Province since 2011 till 2012. DNA was extracted and ferredoxin gene was amplified by PCR technique. The ferredoxin gene PCR products were sequenced to determine gene mutations.

Result

SUBMITTER: Heidari S 

PROVIDER: S-EPMC3887241 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature