Amplification of PVT-1 is involved in poor prognosis via apoptosis inhibition in colorectal cancers.

ABSTRACT:

Background

We previously conducted gene expression microarray analyses to identify novel indicators for colorectal cancer (CRC) metastasis and prognosis from which we identified PVT-1 as a candidate gene. PVT-1, which encodes a long noncoding RNA, mapped to chromosome 8q24 whose copy-number amplification is one of the most frequent events in a wide variety of malignant diseases. However, PVT-1 molecular mechanism of action remains unclear.

Methods

We conducted cell proliferation and invasion assays using colorectal cancer cell lines transfected with PVT-1siRNA or negative control siRNA. Gene expression microarray analyses on these cell lines were also carried out to investigate the molecular function of PVT-1. Further, we investigated the impact of PVT-1 expression on the pr

SUBMITTER: Takahashi Y 

PROVIDER: S-EPMC3887297 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature