RNA interference-mediated repression of S6 kinase 1 impairs root nodule development in soybean.

ABSTRACT: Symbiotic nodule formation on legume roots is characterized with a series of developmental reprograming in root tissues, including extensive proliferation of cortical cells. We examined a possible involvement of the target of rapamycin (TOR) pathway, a central regulator of cell growth and proliferation in animals and yeasts, during soybean nodule development. Our results show that transcription of both GmTOR and its key downstream effector, GmS6K1, are activated during nodulation, which is paralleled with higher kinase activities of these gene products as well. RNAi-mediated knockdown of GmS6K1 impaired the nodule development with severely reduced nodule weight and numbers. In addition, expression of a few nodulins including leghemoglobin was also decreased, and consequently nitrogen fixat

SUBMITTER: Um JH 

PROVIDER: S-EPMC3887909 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature