Protein array-based profiling of CSF identifies RBPJ as an autoantigen in multiple sclerosis.

ABSTRACT:

Objective

To profile the reactivity of CSF-derived immunoglobulin from patients with multiple sclerosis (MS) against a large panel of antigens, to identify disease-specific reactivities.

Methods

CSF from subjects with MS with elevated immunoglobulin G and CSF from control subjects presenting with other inflammatory neurologic disease were screened against a protein array consisting of 9,393 proteins. Reactivity to a candidate protein identified using these arrays was confirmed with ELISA and immunocytochemistry.

Results

Autoantibodies against one protein on the array, recombination signal binding protein for immunoglobulin kappa J region (RBPJ), discriminated between patients with MS and controls (p = 0.0052). Using a large validation cohort, we found a higher prevale

SUBMITTER: Querol L 

PROVIDER: S-EPMC3888197 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature