Activation of the lectin pathway of complement in experimental human keratitis with Pseudomonas aeruginosa.

ABSTRACT:

Purpose

Pseudomonas aeruginosa (P. aeruginosa) microbial keratitis (MK) is a sight-threatening disease. Previous animal studies have identified an important contribution of the complement system to the clearance of P. aeruginosa infection of the cornea. Mannose-binding lectin (MBL), a pattern recognition receptor of the lectin pathway of complement, has been implicated in the host defense against P. aeruginosa. However, studies addressing the role of the lectin pathway in P. aeruginosa MK are lacking. Hence, we sought to determine the activity of the lectin pathway in human MK caused by P. aeruginosa.

Methods

Primary human corneal epithelial cells (HCECs) from cadaveric donors were exposed to two different P. aeruginosa strains. Gene expression of interleukin (IL)-6, IL-8, M

SUBMITTER: Osthoff M 

PROVIDER: S-EPMC3888499 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature