The circadian clock in murine chondrocytes regulates genes controlling key aspects of cartilage homeostasis.

ABSTRACT:

Objective

To characterize the circadian clock in murine cartilage tissue and identify tissue-specific clock target genes, and to investigate whether the circadian clock changes during aging or during cartilage degeneration using an experimental mouse model of osteoarthritis (OA).

Methods

Cartilage explants were obtained from aged and young adult mice after transduction with the circadian clock fusion protein reporter PER2::luc, and real-time bioluminescence recordings were used to characterize the properties of the clock. Time-series microarrays were performed on mouse cartilage tissue to identify genes expressed in a circadian manner. Rhythmic genes were confirmed by quantitative reverse transcription-polymerase chain reaction using mouse tissue, primary chondrocytes, and a

SUBMITTER: Gossan N 

PROVIDER: S-EPMC3888512 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature