Air pollution-mediated susceptibility to inflammation and insulin resistance: influence of CCR2 pathways in mice.

ABSTRACT:

Background

Epidemiologic and experimental studies support an association between PM2.5 exposure and insulin resistance (IR). Innate immune cell activation has been suggested to play a role in the pathogenesis of these effects.

Objectives

We sought to evaluate the role of CC-chemokine receptor 2 (CCR2) in PM2.5-mediated inflammation and IR.

Methods

Wild-type C57BL/6 and CCR2-/- male mice were fed a high-fat diet and exposed to either concentrated ambient PM2.5 or filtered air for 17 weeks via a whole-body exposure system. We evaluated glucose tolerance and insulin sensitivity. At euthanasia, blood, spleen, and visceral adipose tissue (VAT) were collected, and inflammatory cells were measured using flow cytometry. We used standard immunoblots, immunohistochemical method

SUBMITTER: Liu C 

PROVIDER: S-EPMC3888572 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature