Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope.

ABSTRACT:

Background

Two-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded indicators have not been investigated.

New method

Here, we have used a compact acousto-optic lens (AOL) two-photon microscope to make high speed [Ca(2+)] measurements from spines and dendrites distributed in 3D with different excitation wavelengths (800-920 nm).

Results

We show simult

SUBMITTER: Fernandez-Alfonso T 

PROVIDER: S-EPMC3889102 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature