Sequence-based discovery of Bradyrhizobium enterica in cord colitis syndrome.

ABSTRACT:

Background

Immunosuppression is associated with a variety of idiopathic clinical syndromes that may have infectious causes. It has been hypothesized that the cord colitis syndrome, a complication of umbilical-cord hematopoietic stem-cell transplantation, is infectious in origin.

Methods

We performed shotgun DNA sequencing on four archived, paraffin-embedded endoscopic colon-biopsy specimens obtained from two patients with cord colitis. Computational subtraction of human and known microbial sequences and assembly of residual sequences into a bacterial draft genome were performed. We used polymerase-chain-reaction (PCR) assays and fluorescence in situ hybridization to determine whether the corresponding bacterium was present in additional patients and controls.

Results

SUBMITTER: Bhatt AS 

PROVIDER: S-EPMC3889161 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature