Serum metabolomic profile and potential biomarkers for severity of fibrosis in nonalcoholic fatty liver disease.

ABSTRACT:

Background

Biomarker for usefulness in diagnosing advanced fibrosis in nonalcoholic fatty liver disease (NAFLD) is expected. In order to discover novel biomarkers for NAFLD and its pathogenesis, we performed matabolomics screening.

Methods

(1) The initial cohort was 44 NAFLD patients. (2) This validation cohort was 105 NAFLD patients, 26 primary biliary cirrhosis (PBC) patients, and 48 healthy controls. Using capillary electrophoresis and liquid chromatography with mass spectrometry, we analyzed low molecular weight metabolites in these groups.

Results

1. In the initial cohort, we found 28 metabolites associated with advanced fibrosis. Among them, 4 sulfated steroids showed the greatest difference. A decrease of dehydroepiandrosterone sulfate (DHEA-S) and 5α-androstan

SUBMITTER: Tokushige K 

PROVIDER: S-EPMC3889284 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature