Circadian yin-yang regulation and its manipulation to globally reprogram gene expression.

ABSTRACT:

Background

The cyanobacterial circadian program exerts genome-wide control of gene expression. KaiC undergoes rhythms of phosphorylation that are regulated by interactions with KaiA and KaiB. The phosphorylation status of KaiC is thought to mediate global transcription via output factors SasA, CikA, LabA, RpaA, and RpaB. Overexpression of kaiC has been reported to globally repress gene expression.

Results

Here, we show that the positive circadian component KaiA upregulates "subjective dusk" genes and that its overexpression deactivates rhythmic gene expression without significantly affecting growth rates in constant light. We analyze the global patterns of expression that are regulated by KaiA versus KaiC and find in contrast to the previous report of KaiC repression that th

SUBMITTER: Xu Y 

PROVIDER: S-EPMC3889637 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature