E2A predicts prognosis of colorectal cancer patients and regulates cancer cell growth by targeting miR-320a.

ABSTRACT:

Background and objective

Transcriptional factor E2A is crucial for the normal development and differentiation of B and T lymphocytes. Dysregulation of E2A leads to leukemia and tumorigenesis of some solid tumors. The expression and clinical significance of E2A as well as its role in colorectal cancer (CRC) are still unknown. This study aims to assess E2A expression in CRC tissues, evaluate its prognosis value, and investigate its role in colon cancer cell growth.

Methods

E2A expression in CRC tissues and normal mucosa was detected by immunohistochemical staining; Kaplan-Meier survival curve and Cox regression model were used to evaluate the prognostic value of E2A. Lentivirus was used to construct E2A stably knocked-down cells. MTT assay was employed to detect cell prolifera

SUBMITTER: Huang A 

PROVIDER: S-EPMC3890311 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature