Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells.

ABSTRACT: The structural maintenance of chromosomes (SMC) proteins constitute the core of critical complexes involved in structural organization of chromosomes. In yeast, the Smc5/6 complex is known to mediate repair of DNA breaks and replication of repetitive genomic regions, including ribosomal DNA loci and telomeres. In mammalian cells, which have diverse genome structure and scale from yeast, the Smc5/6 complex has also been implicated in DNA damage response, but its further function in unchallenged conditions remains elusive. In this study, we addressed the behavior and function of Smc5/6 during the cell cycle. Chromatin fractionation, immunofluorescence, and live-cell imaging analyses indicated that Smc5/6 associates with chromatin during interphase but largely dissociates from chromosomes whe

SUBMITTER: Gallego-Paez LM 

PROVIDER: S-EPMC3890350 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature