New mini- zincin structures provide a minimal scaffold for members of this metallopeptidase superfamily.

ABSTRACT:

Background

The Acel_2062 protein from Acidothermus cellulolyticus is a protein of unknown function. Initial sequence analysis predicted that it was a metallopeptidase from the presence of a motif conserved amongst the Asp-zincins, which are peptidases that contain a single, catalytic zinc ion ligated by the histidines and aspartic acid within the motif (HEXXHXXGXXD). The Acel_2062 protein was chosen by the Joint Center for Structural Genomics for crystal structure determination to explore novel protein sequence space and structure-based function annotation.

Results

The crystal structure confirmed that the Acel_2062 protein consisted of a single, zincin-like metallopeptidase-like domain. The Met-turn, a structural feature thought to be important for a Met-zincin because it st

SUBMITTER: Trame CB 

PROVIDER: S-EPMC3890501 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature