Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique.

ABSTRACT:

Background

The use of RNAseq to resolve the transcriptional organization of an organism was established in recent years and also showed the complexity and dynamics of bacterial transcriptomes. The aim of this study was to comprehensively investigate the transcriptome of the industrially relevant amino acid producer and model organism Corynebacterium glutamicum by RNAseq in order to improve its genome annotation and to describe important features for transcription and translation.

Results

RNAseq data sets were obtained by two methods, one that focuses on 5'-ends of primary transcripts and another that provides the overall transcriptome with an improved resolution of 3'-ends of transcripts. Subsequent data analysis led to the identification of more than 2,000 transcription sta

SUBMITTER: Pfeifer-Sancar K 

PROVIDER: S-EPMC3890552 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature