Which specimens from a museum collection will yield DNA barcodes? A time series study of spiders in alcohol.

ABSTRACT: We report initial results from an ongoing effort to build a library of DNA barcode sequences for Dutch spiders and investigate the utility of museum collections as a source of specimens for barcoding spiders. Source material for the library comes from a combination of specimens freshly collected in the field specifically for this project and museum specimens collected in the past. For the museum specimens, we focus on 31 species that have been frequently collected over the past several decades. A series of progressively older specimens representing these 31 species were selected for DNA barcoding. Based on the pattern of sequencing successes and failures, we find that smaller-bodied species expire before larger-bodied species as tissue sources for single-PCR standard DNA barcoding. Body si

SUBMITTER: Miller JA 

PROVIDER: S-EPMC3890681 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature