Biosynthesis of the active compounds of Isatis indigotica based on transcriptome sequencing and metabolites profiling.

ABSTRACT:

Background

Isatis indigotica is a widely used herb for the clinical treatment of colds, fever, and influenza in Traditional Chinese Medicine (TCM). Various structural classes of compounds have been identified as effective ingredients. However, little is known at genetics level about these active metabolites. In the present study, we performed de novo transcriptome sequencing for the first time to produce a comprehensive dataset of I. indigotica.

Results

A database of 36,367 unigenes (average length = 1,115.67 bases) was generated by performing transcriptome sequencing. Based on the gene annotation of the transcriptome, 104 unigenes were identified covering most of the catalytic steps in the general biosynthetic pathways of indole, terpenoid, and phenylpropanoid. Subsequently

SUBMITTER: Chen J 

PROVIDER: S-EPMC3890716 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature