Translational dynamics revealed by genome-wide profiling of ribosome footprints in Arabidopsis.

ABSTRACT: Translational regulation contributes to plasticity in metabolism and growth that enables plants to survive in a dynamic environment. Here, we used the precise mapping of ribosome footprints (RFs) on mRNAs to investigate translational regulation under control and sublethal hypoxia stress conditions in seedlings of Arabidopsis thaliana. Ribosomes were obtained by differential centrifugation or immunopurification and were digested with RNase I to generate footprint fragments that were deep-sequenced. Comparison of RF number and position on genic regions with fragmented total and polysomal mRNA illuminated numerous aspects of posttranscriptional and translational control under both growth conditions. When seedlings were oxygen-deprived, the frequency of ribosomes at the start codon was reduced

SUBMITTER: Juntawong P 

PROVIDER: S-EPMC3890782 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature