Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.

ABSTRACT: Diurnal oscillations of gene expression controlled by the circadian clock underlie rhythmic physiology across most living organisms. Although such rhythms have been extensively studied at the level of transcription and mRNA accumulation, little is known about the accumulation patterns of proteins. Here, we quantified temporal profiles in the murine hepatic proteome under physiological light-dark conditions using stable isotope labeling by amino acids quantitative MS. Our analysis identified over 5,000 proteins, of which several hundred showed robust diurnal oscillations with peak phases enriched in the morning and during the night and related to core hepatic physiological functions. Combined mathematical modeling of temporal protein and mRNA profiles indicated that proteins accumulate with

SUBMITTER: Mauvoisin D 

PROVIDER: S-EPMC3890886 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature