The percentage of FoxP3+Helios+ Treg cells correlates positively with disease activity in systemic lupus erythematosus.

ABSTRACT:

Objective

To assess the use of Helios in combination with FoxP3 as a superior method for identifying non-cytokine-producing human Treg cells in patients with systemic lupus erythematosus (SLE) and to determine if FoxP3+Helios+ Treg cells are maintained at normal levels in patients with clinically active disease.

Methods

Peripheral blood mononuclear cells (PBMCs) were purified from the blood of healthy volunteer donors and from 52 consecutive patients with SLE of varying clinical activity (Systemic Lupus Erythematosus Disease Activity Index scores of 0, 2-4, and ≥ 5). PBMCs (either fresh or after 4 hours of stimulation for cytokine production) were then analyzed by flow cytometry for the expression of cell surface markers (CD4, CD25, CD127, and CD45RA) and transcription facto

SUBMITTER: Golding A 

PROVIDER: S-EPMC3891045 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature