Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus.

ABSTRACT:

Background

Lutzomyia gomezi (Nitzulescu, 1931) is one of the main Leishmania (Vianna) panamensis vectors in Panama, and despite its medical significance, there are no population genetic studies regarding this species. In this study, we used the sequences of the mitochondrial gene cytochrome b/start of NADH1 and the nuclear elongation gene α-1 in order to analyze genetic variation and phylogeographic structure of the Lu. gomezi populations.

Methods

A total of 86 Lu. gomezi individuals were captured in 38 locations where cutaneous leishmaniasis occurred. DNA was extracted with phenol/chloroform methods and amplification of genes was performed using PCR primers for mitochondrial and nuclear markers.

Results

We found a total of 37 and 26 haplotypes of mitochondrial and nu

SUBMITTER: Valderrama A 

PROVIDER: S-EPMC3892078 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature