Parthenolide induces apoptosis via TNFRSF10B and PMAIP1 pathways in human lung cancer cells.

ABSTRACT:

Background

Parthenolide (PTL) is a sesquiterpene lactone which can induce apoptosis in cancer cells and eradicate cancer stem cells such as leukemia stem cells, prostate tumor-initiating cells and so on. However, the mechanism remains largely unclear.

Methods

Lung cancer cells were treated with parthenolide and the cell lysates were prepared to detect the given proteins by Western Blot analysis, and the cell survival was assayed by SRB and MTT assay. Cell cycle was evaluated by DNA flow cytometry analysis. TNFRSF10B, PMAIP1, ATF4 and DDIT3 genes were knocked down by siRNA technique. Apoptosis was evaluated by using Annexin V-FITC/PI staining and flow cytometry analysis.

Results

Parthenolide (PTL) induces apoptosis and cell cycle arrest in human lung cancer cells. More

SUBMITTER: Zhao X 

PROVIDER: S-EPMC3892099 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature