Unique DNA methylation loci distinguish anatomic site and HPV status in head and neck squamous cell carcinoma.

ABSTRACT:

Purpose

We have used a genome-wide approach to identify novel differentially methylated CpG dinucleotides that are seen in different anatomic sites of head and neck squamous cell carcinoma (HNSCC), as well as those that might be related to HPV status in the oropharynx.

Experimental design

We conducted genome-wide DNA methylation profiling of primary tumor samples and corresponding adjacent mucosa from 118 HNSCC patients undergoing treatment at Montefiore Medical Center, Bronx, NY, using the Illumina HumanMethylation27 beadchip. For each matched tissue set, we measured differentially methylated CpG loci using a change in methylation level (M-value).

Results

When datasets were individually analyzed by anatomic site of the primary tumor, we identified 293 differentially

SUBMITTER: Lleras RA 

PROVIDER: S-EPMC3892374 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature