Genome-wide methylation analysis and epigenetic unmasking identify tumor suppressor genes in hepatocellular carcinoma.

ABSTRACT:

Background & aims

Epigenetic silencing of tumor suppressor genes contributes to the pathogenesis of hepatocellular carcinoma (HCC). To identify clinically relevant tumor suppressor genes silenced by DNA methylation in HCC, we integrated DNA methylation data from human primary HCC samples with data on up-regulation of gene expression after epigenetic unmasking.

Methods

We performed genome-wide methylation analysis of 71 human HCC samples using the Illumina HumanBeadchip27K array; data were combined with those from microarray analysis of gene re-expression in 4 liver cancer cell lines after their exposure to reagents that reverse DNA methylation (epigenetic unmasking).

Results

Based on DNA methylation in primary HCC and gene re-expression in cell lines after epigenetic

SUBMITTER: Revill K 

PROVIDER: S-EPMC3892430 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature