Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell-free assay.

ABSTRACT: Vesicle transport sorts proteins between compartments and is thereby responsible for generating the non-uniform protein distribution along the eukaryotic secretory and endocytic pathways. The mechanistic details of specific vesicle targeting are not yet well characterized at the molecular level. We have developed a cell-free assay that reconstitutes vesicle targeting utilizing the recycling of resident enzymes within the Golgi apparatus. The assay has physiological properties, and could be used to show that the two lobes of the conserved oligomeric Golgi tethering complex play antagonistic roles in trans-Golgi vesicle targeting. Moreover, we can show that the assay is sensitive to several different congenital defects that disrupt Golgi function and therefore cause glycosylation disorders.

SUBMITTER: Cottam NP 

PROVIDER: S-EPMC3892563 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature