An automated immunoassay for early specificity profiling of antibodies.

ABSTRACT: Antibody-based therapeutics are of great value for the treatment of human diseases. In addition to functional activity, affinity or physico-chemical properties, antibody specificity is considered to be one of the most crucial attributes for safety and efficacy. Consequently, appropriate studies are required before entering clinical trials. High content protein arrays are widely applied to assess antibody specificity, but this commercial solution can only be applied to final therapeutic antibody candidates because such arrays are expensive and their throughput is limited. A flexible, high-throughput and economical assay that allows specificity testing of IgG or Fab molecules during early discovery is described here. The 384-well microtiter plate assay contains a comprehensive panel of 32 te

SUBMITTER: Frese K 

PROVIDER: S-EPMC3893238 | biostudies-literature | 2013 Mar-Apr

REPOSITORIES: biostudies-literature