Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons.

ABSTRACT: The relationship between RNA expression and cell function can often be difficult to decipher due to the presence of both temporal and sub-cellular processing of RNA. These intricacies of RNA regulation can often be overlooked when only acquiring global measurements of RNA expression. This has led to development of several tools that allow for the real-time imaging of individual engineered RNA transcripts in living cells. Here, we describe a new technique that utilizes an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), to image RNA transcripts that were engineered to contain 96-tandem repeats of the RBMB target sequence in the 3'-untranslated region. Binding of RBMBs to the target RNA resulted in discrete bright fluorescent spots, representing individual transcripts, tha

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3893274 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature