Genome-wide analysis of DNA methylation in bovine placentas.

ABSTRACT:

Background

DNA methylation is an important epigenetic modification that is essential for epigenetic gene regulation in development and disease. To date, the genome-wide DNA methylation maps of many organisms have been reported, but the methylation pattern of cattle remains unknown.

Results

We showed the genome-wide DNA methylation map in placental tissues using methylated DNA immunoprecipitation combined with high-throughput sequencing (MeDIP-seq). In cattle, the methylation levels in the gene body are relatively high, whereas the promoter remains hypomethylated. We obtained thousands of highly methylated regions (HMRs), methylated CpG islands, and methylated genes from bovine placenta. DNA methylation levels around the transcription start sites of genes are negatively corre

SUBMITTER: Su J 

PROVIDER: S-EPMC3893433 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature