A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803.

ABSTRACT:

Background

The cyanobacterium Synechocystis sp. PCC 6803 is widely used for research on photosynthesis and circadian rhythms, and also finds application in sustainable biotechnologies. Synechocystis is naturally transformable and undergoes homologous recombination, which enables the development of a variety of tools for genetic and genomic manipulations. To generate multiple gene deletions and/or replacements, marker-less manipulation methods based on counter-selection are generally employed. Currently available methods require two transformation steps with different DNA plasmids.

Results

In this study, we present a marker-less gene deletion and replacement strategy in Synechocystis sp. PCC 6803 which needs only a single transformation step. The method utilizes an nptI-sacB

SUBMITTER: Viola S 

PROVIDER: S-EPMC3893515 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature