A comprehensive tRNA deletion library unravels the genetic architecture of the tRNA pool.

ABSTRACT: Deciphering the architecture of the tRNA pool is a prime challenge in translation research, as tRNAs govern the efficiency and accuracy of the process. Towards this challenge, we created a systematic tRNA deletion library in Saccharomyces cerevisiae, aimed at dissecting the specific contribution of each tRNA gene to the tRNA pool and to the cell's fitness. By harnessing this resource, we observed that the majority of tRNA deletions show no appreciable phenotype in rich medium, yet under more challenging conditions, additional phenotypes were observed. Robustness to tRNA gene deletion was often facilitated through extensive backup compensation within and between tRNA families. Interestingly, we found that within tRNA families, genes carrying identical anti-codons can contribute differently

SUBMITTER: Bloom-Ackermann Z 

PROVIDER: S-EPMC3894157 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature