Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening.

ABSTRACT: Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractive index and to cell thickness. DHM is able to distinguish cellular morphological changes on two representative cell lines (HeLa and H9c2) when treated with doxorubicin and chloroquine, two cytotoxic compounds yielding distinct phenotypes. We analyzed parameters linked to cell morphology and to the intracellular content in endpoint measurements and further investigated them with timelapse recording. The results obtained by DHM were compared with other optical label-free microscopy techniques, namely Phase Contrast, Differential Interferenc

SUBMITTER: Rappaz B 

PROVIDER: S-EPMC3894694 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature