Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity.

ABSTRACT: Obesity and metabolic syndrome is increasing health problem worldwide. Among other ways, nutritional intervention using phytochemicals is important method for treatment and prevention of this disease. Recent studies have shown that certain phytochemicals could alter the expression of specific genes and microRNAs (miRNAs) that play a fundamental role in the pathogenesis of obesity. For study of the obesity and its treatment, monosodium glutamate (MSG)-injected mice with developed central obesity, insulin resistance and liver lipid accumulation are frequently used animal models. To understand the mechanism of phytochemicals action in obese animals, the study of selected genes expression together with miRNA quantification is extremely important. For this purpose, real-time quantitative PCR is

SUBMITTER: Matouskova P 

PROVIDER: S-EPMC3895018 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature