NCoR controls glioblastoma tumor cell characteristics.

ABSTRACT:

Background

We have previously shown that the transcriptional coregulator NCoR represses astrocytic differentiation of neural stem cells, suggesting that NCoR could be a plausible target for differentiation therapy of glioblastoma.

Methods

To study a putative role for NCoR in regulating glioblastoma cell characteristics, we used RNA-mediated knockdown followed by analysis of gene expression, proliferation and cell growth, autophagy, invasiveness in vitro, and tumor formation in vitro and in vivo. We further performed chromatin immunoprecipitation of NCoR followed by genome-wide sequencing in the human glioblastoma cell line U87 in order to reveal NCoR-occupied loci.

Results

RNA knockdown of NCoR resulted in a moderate increase in differentiation accompanied by a signif

SUBMITTER: Heldring N 

PROVIDER: S-EPMC3895387 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature