Longitudinal confocal microscopy imaging of solid tumor destruction following adoptive T cell transfer.

ABSTRACT: A fluorescence-based, high-resolution imaging approach was used to visualize longitudinally the cellular events unfolding during T cell-mediated tumor destruction. The dynamic interplay of T cells, cancer cells, cancer antigen loss variants, and stromal cells-all color-coded in vivo-was analyzed in established, solid tumors that had developed behind windows implanted on the backs of mice. Events could be followed repeatedly within precisely the same tumor region-before, during and after adoptive T cell therapy-thereby enabling for the first time a longitudinal in vivo evaluation of protracted events, an analysis not possible with terminal imaging of surgically exposed tumors. T cell infiltration, stromal interactions, and vessel destruction, as well as the functional consequences thereof,

SUBMITTER: Schietinger A 

PROVIDER: S-EPMC3895414 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature