Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.

ABSTRACT:

Background and objective

Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission.

Materials and methods

To detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and compared factors influencing culture and real-time PCR results.

Results

Totally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling.

Conclusion

The real-time PCR is superior

SUBMITTER: Nikbin VS 

PROVIDER: S-EPMC3895556 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature