The expression and role of serotonin receptor 5HTR2A in canine osteoblasts and an osteosarcoma cell line.

ABSTRACT:

Background

The significance of the serotonergic system in bone physiology and, more specifically, the importance of the five hydroxytryptamine receptor 2A (5HTR2A) in normal osteoblast proliferation have been previously described; however the role of serotonin in osteosarcoma remains unclear. Particularly, the expression and function of 5HTR2A in canine osteosarcoma has not yet been studied, thus we sought to determine if this indoleamine modulates cellular proliferation in vitro. Using real time quantitative reverse transcription PCR and immunoblot analyses, we explored receptor expression and signaling differences between non-neoplastic canine osteoblasts (CnOb) and an osteosarcoma cell line (COS). To elucidate specific serotonergic signaling pathways triggered by 5HTR2A, we perf

SUBMITTER: Bracha S 

PROVIDER: S-EPMC3895671 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature