Telomestatin impairs glioma stem cell survival and growth through the disruption of telomeric G-quadruplex and inhibition of the proto-oncogene, c-Myb.

ABSTRACT:

Purpose

Glioma stem cells (GSC) are a critical therapeutic target of glioblastoma multiforme (GBM).

Experimental design

The effects of a G-quadruplex ligand, telomestatin, were evaluated using patient-derived GSCs, non-stem tumor cells (non-GSC), and normal fetal neural precursors in vitro and in vivo. The molecular targets of telomestatin were determined by immunofluorescence in situ hybridization (iFISH) and cDNA microarray. The data were then validated by in vitro and in vivo functional assays, as well as by immunohistochemistry against 90 clinical samples.

Results

Telomestatin impaired the maintenance of GSC stem cell state by inducing apoptosis in vitro and in vivo. The migration potential of GSCs was also impaired by telomestatin treatment. In contrast, both nor

SUBMITTER: Miyazaki T 

PROVIDER: S-EPMC3896097 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature