Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation.

ABSTRACT:

Background

Gametocyte proteins of Eimeria (E.) spp. are important components of the oocyst wall and some have been used to develop transmission-blocking vaccines against avian coccidiosis.

Methods

Total RNA isolated from E. necatrix gametocytes was utilized as templates for RT-PCR amplification and sequencing of cDNA encoding a gametocyte protein using gene-specific primers. The cDNA was cloned into the bacterial expression vector pET28a(+) and expressed in E. coli BL21 cells. The antigenicity of the recombinant gametocyte protein and its localization in different E. necatrix life-cycle stages were determined by western blot and indirect immunofluorescence analyses, respectively.

Results

A 731-nucleotide sequence of cDNA [GenBank: KF649255] of E. necatrix had 97.7% id

SUBMITTER: Liu D 

PROVIDER: S-EPMC3896727 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature