Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer).

ABSTRACT:

Background

The Asian seabass (Lates calcarifer) is a protandrous hermaphrodite that typically matures as a male at approximately 2-4 years of age and then changes sex in subsequent years. Although several sexual maturation stages have been described histologically for both testis and ovary, the underlying gene expression profiles remain lacking. The development of a gene expression platform is therefore necessary to improve our understanding of the gonad development of this cultured teleost species.

Methods

Thirty Asian seabass gonads were collected from farms in Singapore, examined histologically and staged according to their sex and gonadal maturation status. Partial coding sequences of 24 sex-related genes were cloned using degenerate primers and were sequenced. Additiona

SUBMITTER: Ravi P 

PROVIDER: S-EPMC3896769 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature