A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus.

ABSTRACT:

Background

Peste des petits ruminants (PPR) is an economically important disease of small ruminants such as sheep and goats. The disease is characterized by severe pyrexia, oculo-nasal discharge, pneumonia, necrosis and ulceration of the mucous membrane and inflammation of the gastro-intestinal tract leading to severe diarrhea. A SYBR Green I based real time RT-PCR targeting the N gene of PPRV has not been established for PPRV detection. Thus, the objective of present study was to develop highly sensitive N gene target SYBR Green I real time RT-PCR for specific detection and quantification of PPRV in clinical samples. A set of primers was designed to detect the nucleocapsid (N) gene of PPRV.

Results

The assay exhibited high specificity as all the viruses which have clinical

SUBMITTER: Abera T 

PROVIDER: S-EPMC3896792 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature